Background:

DsbA has a role in cytochrome C synthesis. Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.

Applications:

WB

Name of antibody:

DsbA-Tag

Immunogen:

Fusion protein of E.coli DsbA-Tag

Full name:

periplasmic protein disulfide isomerase I

Synonyms:

dsf; ECK3852; iarA; JW3832; ppfA

SwissProt:

P0AEG4

WB Predicted band size:

23 kDa

WB Positive control:

DsbA with DsbA recombinant protein

WB Recommended dilution:

1000-5000